8JMS

Crystal structure of BelL from Streptomyces cavourensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	62.618, 62.618, 122.464
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.280 - 2.200
R-factor	0.1976
Rwork	0.193
R-free	0.23640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.867
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.280	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.154	0.917
Number of reflections	12865	1096
<I/σ(I)>	14.6	3.3
Completeness [%]	99.1	99
Redundancy	13
CC(1/2)	0.997	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M magnesium diformate, 25% w/v PEG 3350, 0.05M CsCl