8JLY
Structure of nanobody in complex with alpha-synuclein peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-12 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 30.474, 41.906, 86.352 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.500 - 1.290 |
| R-factor | 0.1633 |
| Rwork | 0.161 |
| R-free | 0.18580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.845 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.500 | 1.280 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 30998 | 2028 |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 99.1 | |
| Redundancy | 8.3 | |
| CC(1/2) | 0.980 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M sodium acetate trihydrate pH 4.6, 2.0M ammonium sulfate |
