8JL8
Crystal structure of the collagen binding domain of Cnm from Streptococcus mutans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 40.915, 40.915, 364.893 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.650 - 1.810 |
R-factor | 0.22331 |
Rwork | 0.222 |
R-free | 0.24631 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.840 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.093 | |
Number of reflections | 28413 | 2689 |
<I/σ(I)> | 27.2 | |
Completeness [%] | 99.4 | |
Redundancy | 9.7 | |
CC(1/2) | 0.736 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 22% (w/v) PEG 6000, 100 mM Ammonium sulfate and 10% (v/v) Glycerol |