8JL8

Crystal structure of the collagen binding domain of Cnm from Streptococcus mutans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	40.915, 40.915, 364.893
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.650 - 1.810
R-factor	0.22331
Rwork	0.222
R-free	0.24631
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.840
High resolution limit [Å]	1.810	1.810
Rmerge	0.093
Number of reflections	28413	2689
<I/σ(I)>	27.2
Completeness [%]	99.4
Redundancy	9.7
CC(1/2)		0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	22% (w/v) PEG 6000, 100 mM Ammonium sulfate and 10% (v/v) Glycerol