8JL7

Crystal structure of the Green fluorescent protein SEA227D variant at pH 8.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	193
Detector technology	CCD
Collection date	2022-06-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	41.681, 53.479, 98.187
Unit cell angles	90.00, 91.18, 90.00

Refinement procedure

Resolution	38.650 - 1.760
R-factor	0.2138
Rwork	0.212
R-free	0.24940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.094
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.820
High resolution limit [Å]	1.760	3.790	1.760
Rmerge	0.086	0.060	0.344
Rmeas	0.093	0.065	0.397
Rpim	0.037	0.025	0.193
Total number of observations	218715
Number of reflections	41907	4379	3839
<I/σ(I)>	6.8
Completeness [%]	97.1	98.9	89
Redundancy	5.2	6.8	3.4
CC(1/2)	0.996	0.997	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1M Tris pH 8.0, 0.2M lithium chloride, 20% PEG 6000