8JL2

Crystal structure of the Green fluorescent protein SE_A277 variant at pH 9.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	193
Detector technology	CCD
Collection date	2022-06-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	76.345, 80.548, 179.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.120 - 1.760
R-factor	0.1911
Rwork	0.191
R-free	0.21760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.121
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.810
High resolution limit [Å]	1.750	3.770	1.750
Rmerge	0.095	0.051	0.434
Rmeas	0.100	0.053	0.468
Rpim	0.028	0.014	0.168
Total number of observations	1160484
Number of reflections	109847	11700	9194
<I/σ(I)>	7.2
Completeness [%]	97.7	99.4	83
Redundancy	10.6	13	6.8
CC(1/2)	0.997	0.999	0.572

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1M sodium Tris pH 9.5, 0.8M sodium dihydrogen phosphate