8JKL

IRF4 DNA-binding domain bound to an DNA containing GATA motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9752
Spacegroup name	P 31 2 1
Unit cell lengths	117.560, 117.560, 150.510
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.480 - 2.940
R-factor	0.2428
Rwork	0.241
R-free	0.27620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jm4
RMSD bond length	0.003
RMSD bond angle	1.049
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.480	3.020
High resolution limit [Å]	2.940	2.940
Rmerge	0.228	1.923
Rmeas	0.234	1.976
Rpim	0.052	0.449
Total number of observations	516859	36412
Number of reflections	26113	1894
<I/σ(I)>	14	2
Completeness [%]	99.9
Redundancy	19.8	19.2
CC(1/2)	0.998	0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M MgCl2, 0.1 M sodium citrate (pH 5.0) and 15% (w/v) PEG 4000