8JKI

Crystal structure of the Green fluorescent protein SE_A277 variant at pH 7.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	193
Detector technology	CCD
Collection date	2022-06-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	78.166, 78.369, 179.473
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.550 - 2.100
R-factor	0.2098
Rwork	0.208
R-free	0.26530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.013
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.122	0.077	0.398
Rmeas	0.129	0.080	0.448
Rpim	0.041	0.022	0.197
Total number of observations	450311
Number of reflections	61621	6845	5192
<I/σ(I)>	7.4
Completeness [%]	94.4	99.2	80.3
Redundancy	7.3	11.9	4
CC(1/2)	0.993	0.997	0.390

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1M HEPES pH 7.5, 2.5M sodium chloride, 12% PEG 1500, 2.2M sodium chloride