8JKG

Crystal structure of the Green fluorescent protein SE_A277 variant at pH 5.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	193
Detector technology	CCD
Collection date	2022-06-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	76.380, 79.981, 179.367
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.570 - 2.200
R-factor	0.1883
Rwork	0.187
R-free	0.22980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.061
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.280
High resolution limit [Å]	2.200	4.740	2.200
Rmerge	0.109	0.076	0.314
Rmeas	0.115	0.079	0.349
Rpim	0.035	0.022	0.147
Total number of observations	447004
Number of reflections	54987	5854	5233
<I/σ(I)>	6.8
Completeness [%]	97.4	98	94.4
Redundancy	8.1	11.7	4.7
CC(1/2)	0.993	0.997	0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	0.1M sodium acetate pH 5.5), 2.2M Sodium Chloride