8JJV
Structure of truncated form of nanobody in complex with alpha-synuclein peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-20 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 30.346, 41.618, 85.032 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.230 |
| R-factor | 0.1452 |
| Rwork | 0.142 |
| R-free | 0.16990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.883 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.260 |
| High resolution limit [Å] | 1.200 | 1.230 |
| Rmerge | 0.080 | 0.470 |
| Number of reflections | 31814 | 2048 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 98.8 | |
| Redundancy | 7.5 | |
| CC(1/2) | 0.990 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M BIS-TRIS pH 5.5, 2.0M ammonium sulfate |
