8JJQ
Structure of SenB in complex with UDP-GalNAc at 1.64 Angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.114, 56.377, 108.130 |
| Unit cell angles | 90.00, 91.01, 90.00 |
Refinement procedure
| Resolution | 49.990 - 1.640 |
| R-factor | 0.17691 |
| Rwork | 0.176 |
| R-free | 0.19542 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.968 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.990 | 1.670 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Number of reflections | 157933 | 6787 |
| <I/σ(I)> | 13.2 | 2.2 |
| Completeness [%] | 98.8 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.997 | 0.655 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.153 | 20% PEG 5000-MME, 0.1M Bis-Tris pH 6.5 |
