8JJN
Structure of SenB in complex with UDP-Glc and PO4- at 1.98 Angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.825, 55.792, 108.076 |
| Unit cell angles | 90.00, 90.94, 90.00 |
Refinement procedure
| Resolution | 48.550 - 1.980 |
| R-factor | 0.1925 |
| Rwork | 0.191 |
| R-free | 0.21990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.977 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.570 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 89982 | 575 |
| <I/σ(I)> | 12.7 | 1.9 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.998 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.153 | 20%PEG8000, 0.2M(NH4SO4, 0.1M Hepes pH 7.5,10% 2-propanol |
