8JJC
Tubulin-Y62
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97777 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.493, 157.005, 180.398 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.740 - 2.760 |
| R-factor | 0.2357 |
| Rwork | 0.235 |
| R-free | 0.26430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.680 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.730 | 2.890 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Number of reflections | 1818003 | 25474 |
| <I/σ(I)> | 7.62 | |
| Completeness [%] | 99.0 | |
| Redundancy | 10.4 | |
| CC(1/2) | 0.830 | 0.120 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, 30 mM MgCl2 |
