8JJ9

Human FAM91A1 N terminal domain in complex with TBC1D23

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	88.212, 93.558, 111.514
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.010 - 2.510
R-factor	0.2252
Rwork	0.225
R-free	0.27660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.136
Data reduction software	autoPROC
Data scaling software	DIALS
Phasing software	SHELXS
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	111.510	2.580
High resolution limit [Å]	2.510	2.510
Rmerge	0.145
Rpim		0.885
Number of reflections	32278	2358
<I/σ(I)>	11.6	1.2
Completeness [%]	100.0	100
Redundancy	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289.15	Tetraethylene glycol, Pentaethylene glycol, PH8.5 Tris, Ethylene glycol, PEG 8000