8JHH

Glycoside hydrolase family 55 endo-beta-1,3-glucanase from Microdochium nivale

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	I 21 21 21
Unit cell lengths	89.200, 211.003, 211.175
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.950 - 2.400
R-factor	0.1744
Rwork	0.163
R-free	0.19350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.628
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.550
High resolution limit [Å]	2.400	2.400
Number of reflections	77596	12330
<I/σ(I)>	6.48
Completeness [%]	99.8
Redundancy	6.03
CC(1/2)	0.981	0.588

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M HEPES-NaOH buffer (pH 7.0), 1 M disodium succinate, 10 mg/mL polyethylene glycol monomethyl ether 2000, 10 mM glucose