8JHE

Hyper-thermostable ancestral L-amino acid oxidase 2 (HTAncLAAO2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-26
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00
Spacegroup name	P 4
Unit cell lengths	180.896, 180.896, 81.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.500 - 2.201
Rwork	0.167
R-free	0.20010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.518
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	2.350
High resolution limit [Å]	2.200	2.200
Number of reflections	133518	19360
<I/σ(I)>	21.5	5.3
Completeness [%]	100.0
Redundancy	20
CC(1/2)	0.999	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	10% PEG3350, 0.2 M ammonium citrate tribasic, and 4%(v/v) 1,1,1,3,3,3-Hexafluoro-2-propanol