8JG7
Serine decarboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.360, 149.450, 139.126 |
| Unit cell angles | 90.00, 109.18, 90.00 |
Refinement procedure
| Resolution | 49.390 - 2.850 |
| R-factor | 0.21591 |
| Rwork | 0.214 |
| R-free | 0.26180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.410 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.850 | 7.730 | 2.850 |
| Rmerge | 0.173 | 0.165 | 0.551 |
| Rmeas | 0.189 | 0.180 | 0.616 |
| Rpim | 0.076 | 0.070 | 0.270 |
| Number of reflections | 52125 | 2685 | 2556 |
| <I/σ(I)> | 3.5 | ||
| Completeness [%] | 99.6 | 100 | 98.1 |
| Redundancy | 5.9 | 6.4 | 5 |
| CC(1/2) | 0.860 | 0.904 | 0.846 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 20% PEG 400, 0.2M CaCl2, PH8.0 |
