8JEB

Crystal structure of CGL1 from Crassostrea gigas, mannotetraose-bound form (CGL1/Man(alpha)1-2Man(alpha)1-2Man(alpha)1-6Man)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	90
Detector technology	PIXEL
Collection date	2021-11-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	76.400, 82.495, 44.201
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.201 - 1.300
Rwork	0.156
R-free	0.17450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.655
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.201	1.320
High resolution limit [Å]	1.300	1.300
Rmerge	0.053	0.747
Number of reflections	68918	3007
<I/σ(I)>	22.9
Completeness [%]	99.2
Redundancy	12.5
CC(1/2)	1.000	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2 M magnesium acetate, 0.1 M sodium cacodylate (pH 6.5), 20% PEG 8000