8JDH

Crystal structure of anti-CRISPR AcrIF25

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97852
Spacegroup name	C 1 2 1
Unit cell lengths	85.953, 52.289, 85.121
Unit cell angles	90.00, 105.91, 90.00

Refinement procedure

Resolution	44.190 - 1.770
R-factor	0.169
Rwork	0.168
R-free	0.19200
Structure solution method	SAD
Data reduction software	DENZO
Data scaling software	HKL-3000
Phasing software	AutoSol
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.770	1.770
Number of reflections	35124	1605
<I/σ(I)>	27.3
Completeness [%]	98.7
Redundancy	13.1
CC(1/2)	0.996	0.937

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	289	0.1 M SODIUM ACETATE, PH 5.0, 11 % MPD, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 289K