8JDC

Crystal structure of mLDHD in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-28
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9792
Spacegroup name	I 2 2 2
Unit cell lengths	81.486, 103.162, 122.591
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.682 - 1.696
R-factor	0.1795
Rwork	0.179
R-free	0.19410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.955
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.930	1.790
High resolution limit [Å]	1.696	1.700
Rmerge	0.054	0.672
Rmeas	0.058	0.731
Rpim	0.021	0.284
Total number of observations		52341
Number of reflections	51046	8242
<I/σ(I)>	18.8	2.8
Completeness [%]	88.9
Redundancy	7.2	6.4
CC(1/2)	0.999	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	4.0 M sodium formate