8JD7
endo-1,3-fucanase GH174A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.176, 70.496, 103.003 |
| Unit cell angles | 90.00, 107.53, 90.00 |
Refinement procedure
| Resolution | 28.220 - 1.600 |
| R-factor | 0.1552 |
| Rwork | 0.155 |
| R-free | 0.19110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.889 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.110 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.041 | 0.198 |
| Rmeas | 0.051 | 0.255 |
| Rpim | 0.031 | 0.159 |
| Total number of observations | 287357 | 12710 |
| Number of reflections | 121349 | 6039 |
| <I/σ(I)> | 14.8 | 4.3 |
| Completeness [%] | 95.0 | |
| Redundancy | 2.4 | 2.1 |
| CC(1/2) | 0.998 | 0.922 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 100 mM Imidazole/ Hydrochloric acid pH 8.0+200 mM Sodium chloride+30% (w/v) PEG 8000 |
