8JBJ

Crystal structure of anti-PVRIG Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	293
Detector technology	PIXEL
Collection date	2022-04-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	64.268, 76.408, 192.424
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.180 - 1.610
R-factor	0.1864
Rwork	0.184
R-free	0.22550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.827
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	10.000	1.710
High resolution limit [Å]	1.610	4.820	1.610
Rmerge	0.117	0.072	0.767
Rmeas	0.127	0.078	0.909
Number of reflections	122876	8840	15300
<I/σ(I)>	12.16
Completeness [%]	84.6
Redundancy	5.7
CC(1/2)	0.997	0.995	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	21% PEG3350, 0.4 M Potassium Formate