8JB1

Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium glutamicum ATCC13032 in complex with NADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2023-04-29
Detector	ADSC QUANTUM 270
Wavelength(s)	0.987
Spacegroup name	P 43 2 2
Unit cell lengths	92.754, 92.754, 287.554
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.540 - 2.440
R-factor	0.19589
Rwork	0.192
R-free	0.26249
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.608
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.440	6.650	2.450
Rmerge		0.062	0.295
Rmeas		0.065	0.326
Rpim		0.019	0.133
Number of reflections	45137	2543	2089
<I/σ(I)>	13.8
Completeness [%]	94.7	95	90.9
Redundancy	8.3	10.3	4.9
CC(1/2)	0.795	0.997	0.795

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	PEG 3K, HEPES/NaOH, sodium chloride