8JAI

Crystal Structure of Human H-Ferritin variant 123F assembling in solution 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2022-12-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	I 41 2 2
Unit cell lengths	301.660, 301.660, 316.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.590 - 2.560
Rwork	0.335
R-free	0.36840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.289
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.590	2.650
High resolution limit [Å]	2.560	2.560
Number of reflections	231274	22913
<I/σ(I)>	7.56
Completeness [%]	99.9
Redundancy	2
CC(1/2)	0.996	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9.5	293	50 mM CAPS pH 9.5, 200 mM Sodium chloride