8J9M
Crystal Structure of Human H-Ferritin variant 123F assembling in solution3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 193 |
| Detector technology | CMOS |
| Collection date | 2022-11-17 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 0.987 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 143.331, 143.331, 166.663 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.570 - 2.900 |
| R-factor | 0.2051 |
| Rwork | 0.203 |
| R-free | 0.24340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.158 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.570 | 3.004 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 19523 | 1684 |
| <I/σ(I)> | 45.7 | |
| Completeness [%] | 98.3 | |
| Redundancy | 1.1 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 50 mM TRIS-HCL pH 7.5, 200 mM Sodium chloride |
