8J9L

Crystal Structure of Human H-Ferritin variant 123F assembling in solution2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2022-11-19
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.987
Spacegroup name	H 3 2
Unit cell lengths	261.696, 261.696, 320.511
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.260 - 2.500
R-factor	0.2478
Rwork	0.247
R-free	0.28910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.098
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.260	2.589
High resolution limit [Å]	2.500	2.500
Number of reflections	144447	14354
<I/σ(I)>	4
Completeness [%]	97.2
Redundancy	2
CC(1/2)	0.412	0.702

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9.5	293	50 mM BICINE pH 9.5, 800 mM sodium chloride