8J8T
Crystal structure of calcium-saturated SRCR domain 11 of DMBT1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-24 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.197, 47.305, 69.833 |
| Unit cell angles | 90.00, 101.85, 90.00 |
Refinement procedure
| Resolution | 38.900 - 1.810 |
| R-factor | 0.16952 |
| Rwork | 0.166 |
| R-free | 0.23341 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8j8d |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.313 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 5.370 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Number of reflections | 35273 | 5540 |
| <I/σ(I)> | 10.61 | |
| Completeness [%] | 92.5 | |
| Redundancy | 1.79 | |
| CC(1/2) | 0.997 | 0.933 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 0.2 M Calcium acetate hydrate, 20% Polyethylene glycol 3350 |
