8J7E
Crystal structure of BRIL in complex with 1b3 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 116.319, 116.319, 212.326 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.010 - 2.800 |
R-factor | 0.2022 |
Rwork | 0.200 |
R-free | 0.25050 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.812 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.010 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 1.546 | |
Rpim | 0.352 | |
Number of reflections | 41670 | 4117 |
<I/σ(I)> | 11.32 | |
Completeness [%] | 99.9 | |
Redundancy | 20 | |
CC(1/2) | 0.996 | 0.768 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 295.15 | 0.1 M sodium acetate pH 5.0, 15% (w/v) PEG 6000 |