8J72

Crystal structure of mammalian Trim71 in complex with lncRNA Trincr1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2019-10-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97890
Spacegroup name	P 1
Unit cell lengths	45.529, 51.450, 73.154
Unit cell angles	77.85, 75.87, 77.64

Refinement procedure

Resolution	37.580 - 3.160
Rwork	0.215
R-free	0.28140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fpt
RMSD bond length	0.004
RMSD bond angle	0.880
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.260
High resolution limit [Å]	3.160	3.160
Number of reflections	10186	492
<I/σ(I)>	10.14
Completeness [%]	98.1
Redundancy	3.8
CC(1/2)	0.988	0.908

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	0.1 M Ammonium tartrate dibasic pH7.0,12% PEG 3350 (w/v)