8J70

Native SAND domain of protein SP140 with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	110
Detector technology	PIXEL
Collection date	2020-04-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	76.629, 119.071, 64.679
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.420 - 1.850
R-factor	0.2023
Rwork	0.201
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.877
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.880
High resolution limit [Å]	1.850	5.020	1.850
Rmerge	0.067	0.048	0.699
Rmeas	0.070	0.051	0.729
Rpim	0.020	0.015	0.206
Total number of observations	312594
Number of reflections	25564	1370	1258
<I/σ(I)>	9.9
Completeness [%]	99.8	98.7	100
Redundancy	12.2	11.4	12
CC(1/2)	0.993	0.998	0.944

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	6% v/v Tacsimate pH 6.0, 22% w/v PEG 3350, 10 mM FeCl3