8J69
Crystal structure of HORMA domain-containing protein 1 (HORMAD1) from Homo sapiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.367, 64.498, 81.643 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.610 - 2.670 |
| R-factor | 0.2084 |
| Rwork | 0.204 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.013 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.610 | 2.740 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmerge | 0.120 | |
| Number of reflections | 15433 | 3844 |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6 | 6.4 |
| CC(1/2) | 0.972 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 4% TacsimateTM PH6.0, 12% (w/v) Polyethylene |
