8J56

Crystal structure of the FlhDC complex from Cupriavidus necator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-11
Detector	ADSC QUANTUM 270
Wavelength(s)	1.000
Spacegroup name	P 32 2 1
Unit cell lengths	124.533, 124.533, 173.853
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.910 - 3.500
R-factor	0.2219
Rwork	0.220
R-free	0.26130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.578
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.560
High resolution limit [Å]	3.500	3.500
Number of reflections	20112	989
<I/σ(I)>	9.8	1.8
Completeness [%]	100.0	100
Redundancy	5.3	5.4
CC(1/2)	0.970	0.358

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	PEG 8000, magnesium chloride