8J50
Crystal structure of Flavihumibacter petaseus GH31 alpha-galactosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-15 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.662, 72.364, 81.074 |
| Unit cell angles | 101.74, 104.95, 103.66 |
Refinement procedure
| Resolution | 44.415 - 1.900 |
| Rwork | 0.137 |
| R-free | 0.18080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.620 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.090 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.079 | 0.437 |
| Rmeas | 0.112 | 0.618 |
| Rpim | 0.079 | 0.437 |
| Number of reflections | 111441 | 4433 |
| <I/σ(I)> | 6.4 | 1.5 |
| Completeness [%] | 96.4 | 77.4 |
| Redundancy | 3.5 | 3.3 |
| CC(1/2) | 0.995 | 0.770 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1 M sodium chloride, 50 mM MES-NaOH |
