8J4C

YeeE(TsuA)-YeeD(TsuB) complex for thiosulfate uptake

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 21 2 21
Unit cell lengths	73.980, 102.440, 186.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.390 - 3.340
R-factor	0.2691
Rwork	0.265
R-free	0.31080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.565
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.390	3.470
High resolution limit [Å]	3.340	3.350
Rmeas	1.095	4.632
Rpim	0.281	1.012
Number of reflections	21071	1989
<I/σ(I)>	4.15	0.85
Completeness [%]	99.1	97.31
Redundancy	20.4	18.5
CC(1/2)	0.964	0.559

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7	293	18-24% Pentaerythritol-propoxylate (5/4 PO/OH), 100 mM 2-morpholinoethanesulfonic acid (MES)-NaOH (pH 7.0), 300 mM NaCl