8J4A
Crystal structure of OY phytoplasma SAP05 in complex with AtRPN10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2022-09-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.045, 40.586, 130.329 |
| Unit cell angles | 90.00, 93.01, 90.00 |
Refinement procedure
| Resolution | 48.950 - 1.970 |
| R-factor | 0.1954 |
| Rwork | 0.193 |
| R-free | 0.25260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.969 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.950 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.066 | 0.468 |
| Number of reflections | 38214 | 3983 |
| <I/σ(I)> | 11.87 | 2.85 |
| Completeness [%] | 94.6 | |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Lithium acetate dehydrate, 18% PEG3350, 0.1 M Guanine hydrochloride |
