8J49
Crystal structure of OY phytoplasma SAP05 in complex with AtSPL5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-01-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.28277 |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.159, 45.091, 52.947 |
| Unit cell angles | 92.43, 101.86, 108.62 |
Refinement procedure
| Resolution | 42.440 - 1.660 |
| R-factor | 0.1941 |
| Rwork | 0.192 |
| R-free | 0.21970 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.440 | 1.750 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.034 | 0.250 |
| Number of reflections | 25490 | 1455 |
| <I/σ(I)> | 11.7 | 2.3 |
| Completeness [%] | 83.5 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Sodium fluoride, 20% w/v Polyethylene glycol 3,350 |
