8J31

Glucosyl transferase crystallized in the presence of beta carotene

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.987
Spacegroup name	I 4 2 2
Unit cell lengths	155.297, 155.297, 128.529
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.160 - 3.780
R-factor	0.3651
Rwork	0.363
R-free	0.38524
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.954
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (refmac5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.510	4.230
High resolution limit [Å]	3.780	3.780
Number of reflections	8122	2240
<I/σ(I)>	3.6
Completeness [%]	99.6
Redundancy	17.4
CC(1/2)	0.890	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M Magnesium chloride hexahydrate, 0.1 M Tris pH 7.0, 10% w/v PEG 8000