8J2Z
Glucosyl transferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-19 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.987 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 153.384, 153.384, 126.657 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.500 - 2.783 |
| R-factor | 0.2286 |
| Rwork | 0.223 |
| R-free | 0.27680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.172 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.500 | 2.883 |
| High resolution limit [Å] | 2.783 | 2.783 |
| Number of reflections | 19066 | 1570 |
| <I/σ(I)> | 7.64 | |
| Completeness [%] | 98.2 | |
| Redundancy | 25.9 | |
| CC(1/2) | 0.991 | 0.445 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M Magnesium chloride hexahydrate, 0.1 M Sodium HEPES pH 7.5, 30% v/vPEG 400 |
