8J2U

Glucosyl Transferase NbUGT72AY1 co-crystallized with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	I 1 2 1
Unit cell lengths	100.492, 112.559, 103.632
Unit cell angles	90.00, 102.25, 90.00

Refinement procedure

Resolution	45.960 - 3.320
R-factor	0.29196
Rwork	0.289
R-free	0.33038
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.073
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (Refmac5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.960	3.410
High resolution limit [Å]	3.320	3.320
Number of reflections	16627	1133
<I/σ(I)>	3
Completeness [%]	99.2
Redundancy	6.2
CC(1/2)	0.960	0.370

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M Lithium chloride, 0.1 M MES pH 6.0, 20% w/vPEG 6000