8J2T

Glucosyl Transferase NbUGT72AY1 co-crystallized with UDP-2F glucose and Scopoletin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.987
Spacegroup name	I 1 2 1
Unit cell lengths	101.607, 114.919, 104.249
Unit cell angles	90.00, 103.12, 90.00

Refinement procedure

Resolution	46.830 - 3.150
R-factor	0.2
Rwork	0.197
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.900	3.160
High resolution limit [Å]	3.080	3.080
Number of reflections	18992	3327
<I/σ(I)>	4.9
Completeness [%]	97.2
Redundancy	3.6
CC(1/2)	0.984	0.464

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 MLithium sulfate, 0.1 M Tris pH 8.5, 30% w/vPEG 4000