8J2Q

Crystal structure of Cypovirus Polyhedra mutant fused with c-Myc fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	I 2 3
Unit cell lengths	105.970, 105.970, 105.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.470 - 1.920
R-factor	0.2062
Rwork	0.202
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.999
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.840	1.840
Number of reflections	17287	2724
<I/σ(I)>	11.42
Completeness [%]	99.6
Redundancy	87.8
CC(1/2)	0.996	0.584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		293	cell-free crystallization