8J2P

Crystal structure of PML B-box2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	H 3
Unit cell lengths	122.550, 122.550, 163.224
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.380 - 2.090
R-factor	0.1927
Rwork	0.191
R-free	0.23460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.688
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.21rc1_4924: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.400	2.180
High resolution limit [Å]	2.090	2.100
Rpim	0.030
Number of reflections	53556	3559
<I/σ(I)>	33.9
Completeness [%]	99.8
Redundancy	6.6
CC(1/2)	0.997	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	20% PEG 3350