8J2N

Exopolysaccharide phosphotransferase CpsY in Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	79.880, 41.730, 100.440
Unit cell angles	90.00, 93.64, 90.00

Refinement procedure

Resolution	26.100 - 1.640
R-factor	0.204
Rwork	0.203
R-free	0.22640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.851
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.100	1.670
High resolution limit [Å]	1.640	1.640
Rmerge	0.079	1.133
Number of reflections	81599	5998
<I/σ(I)>	13.8
Completeness [%]	99.9
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	0.1 M Citric acid, 0.05 M BIS-TRIS propane, pH 5.8, 15% w/v Polyethylene glycol 3350