8J2N
Exopolysaccharide phosphotransferase CpsY in Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.880, 41.730, 100.440 |
| Unit cell angles | 90.00, 93.64, 90.00 |
Refinement procedure
| Resolution | 26.100 - 1.640 |
| R-factor | 0.204 |
| Rwork | 0.203 |
| R-free | 0.22640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.851 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.100 | 1.670 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.079 | 1.133 |
| Number of reflections | 81599 | 5998 |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | 0.1 M Citric acid, 0.05 M BIS-TRIS propane, pH 5.8, 15% w/v Polyethylene glycol 3350 |
