8J1I
Crystal Structure of EphA8/SASH1 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 45.937, 84.576, 97.804 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.010 - 1.600 |
| R-factor | 0.19882 |
| Rwork | 0.197 |
| R-free | 0.23680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fxf |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.178 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 4.340 | 1.600 |
| Rmerge | 0.047 | 0.036 | 1.212 |
| Rmeas | 0.049 | 0.038 | 1.259 |
| Rpim | 0.014 | 0.011 | 0.340 |
| Total number of observations | 332592 | ||
| Number of reflections | 25509 | 1389 | 1242 |
| <I/σ(I)> | 5.9 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 13 | 12.4 | 13.4 |
| CC(1/2) | 0.995 | 0.999 | 0.777 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | PEG 1500,10% isopropanol |
