8J1G
Structure of amino acid dehydrogenase in complex with NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.473, 70.306, 100.137 |
| Unit cell angles | 88.65, 72.53, 86.53 |
Refinement procedure
| Resolution | 47.800 - 2.500 |
| R-factor | 0.22761 |
| Rwork | 0.225 |
| R-free | 0.27393 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.379 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.800 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.063 | 1.200 |
| Rmeas | 0.068 | 1.300 |
| Rpim | 0.026 | 0.495 |
| Total number of observations | 31137 | |
| Number of reflections | 56308 | 4614 |
| <I/σ(I)> | 18.9 | 1.8 |
| Completeness [%] | 98.7 | |
| Redundancy | 7 | 6.7 |
| CC(1/2) | 0.999 | 0.657 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | PEG 4000, Na acetate buffer, glycerol, NADPH, arginine |
