8J16
Crystal structure of horse spleen L-ferritin at -80deg Celsius.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 193.15 |
| Detector technology | PIXEL |
| Collection date | 2022-12-20 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5420 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 182.016, 182.016, 182.016 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.752 - 1.600 |
| Rwork | 0.176 |
| R-free | 0.20940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.598 |
| Data reduction software | CrysalisPro (v42) |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | MOLREP (11.9.02) |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.700 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.108 | |
| Rmeas | 0.122 | |
| Rpim | 0.055 | |
| Number of reflections | 34413 | 1649 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 99.6 | |
| Redundancy | 4.7 | |
| CC(1/2) | 0.993 | 0.365 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | Ammonium sulfate (0.5-1.0M), Cadmium sulfate(12-20mM) |
