8J11
Crystal structure of horse spleen L-ferritin at 0deg Celsius.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 273.15 |
| Detector technology | PIXEL |
| Collection date | 2022-11-04 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 182.084, 182.084, 182.084 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.091 - 1.600 |
| Rwork | 0.155 |
| R-free | 0.18640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.648 |
| Data reduction software | CrysalisPro (v42) |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | MOLREP (11.9.02) |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.091 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.077 | 0.901 |
| Rmeas | 0.087 | 1.102 |
| Rpim | 0.039 | 0.622 |
| Number of reflections | 34317 | 1619 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 99.3 | 96.7 |
| Redundancy | 4.5 | |
| CC(1/2) | 0.997 | 0.460 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | Ammonium sulfate (0.5-1.0M), Cadmium sulfate(12-20mM) |
