8J0X

Crystal structure of horse spleen L-ferritin at -20deg Celsius.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	253.15
Detector technology	PIXEL
Collection date	2022-11-08
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5420
Spacegroup name	F 4 3 2
Unit cell lengths	182.340, 182.340, 182.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.286 - 1.600
Rwork	0.148
R-free	0.16870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.714
Data reduction software	CrysalisPro (v42)
Data scaling software	Aimless (0.7.9)
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.286	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.065	0.605
Rmeas	0.073	0.732
Rpim	0.031	0.404
Number of reflections	34348	1665
<I/σ(I)>	15.6	1.9
Completeness [%]	98.9
Redundancy	4.7
CC(1/2)	0.998	0.708

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Ammonium sulfate (0.5-1.0M), Cadmium sulfate(12-20mM)