8J0W

Crystal structure of horse spleen L-ferritin at -40deg Celsius.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	233.15
Detector technology	PIXEL
Collection date	2022-12-19
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.542
Spacegroup name	F 4 3 2
Unit cell lengths	181.999, 181.999, 181.999
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.245 - 1.600
Rwork	0.145
R-free	0.17520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.661
Data reduction software	CrysalisPro (v42)
Data scaling software	Aimless (0.7.9)
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.245	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.062	0.442
Rmeas	0.069	0.539
Rpim	0.029	0.303
Number of reflections	34339	1644
<I/σ(I)>	14.7	2.5
Completeness [%]	99.5
Redundancy	4.9
CC(1/2)	0.998	0.801

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293.15	Ammonium sulfate (0.5-1.0M), Cadmium sulfate(12-20mM)