8IYP

Crystal structure of serine palmitoyltransferase soaked in 190 mM D-serine solution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-23
Detector	RAYONIX MX225HE
Wavelength(s)	0.9
Spacegroup name	P 41 21 2
Unit cell lengths	60.975, 60.975, 207.311
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.248 - 1.651
Rwork	0.149
R-free	0.18420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a2b
RMSD bond length	0.010
RMSD bond angle	1.682
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.750
High resolution limit [Å]	1.650	1.650
Number of reflections	47801	67830
<I/σ(I)>	38.7
Completeness [%]	99.2
Redundancy	12.9
CC(1/2)	1.000	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG4000, sodium acetate, Tris-HCl