8IYN

Crystal structure of LOV1 D33N mutant of phototropin from Klebsormidium nitens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.918402
Spacegroup name	P 43 21 2
Unit cell lengths	53.451, 53.451, 138.514
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.867 - 2.081
Rwork	0.170
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.452
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.867	6.622
High resolution limit [Å]	2.080	2.081
Number of reflections	12100	9452
<I/σ(I)>	10.8
Completeness [%]	95.1
Redundancy	20
CC(1/2)	0.997	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	sodium phoshate monobasic monohydrate , potassium phosphate dibasic , pH 8.2